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human ifn β  (InvivoGen)
94
InvivoGen human ifn β
Poxin transgene inhibits DNA sensing-induced innate immune response. A. Schematic of cloning process. B-G. Cells were transfected with plasmid DNA (pDNA) using TransIT-2020. B. 2.5 μg and 7.5 μg of pDNA encoding Poxin or mutant Poxin (mtPoxin) were transfected into 293T cells, respectively, and FLAG-tagged proteins were detected. C. Activation of STING, TBK1, and IRF3 was analyzed by western blotting. Quantitative analysis of p-IRF3 expression level is shown. D. H151 was pre-treated 1 h before pDNA transfection, and protein expression levels were assessed after 24 h. E. Cells were transfected with pEGFP-N1, and protein expression levels were detected. F. mRNA levels of IFNB1 and ISG15 were analyzed by RT-qPCR (n = 3). <t>G.</t> <t>IFN-β</t> secretion levels were quantitatively analyzed using ELISA. (Representative western blots are shown.).
Human Ifn β, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human ifnβ  (Sino Biological)
94
Sino Biological human ifnβ
HSPA6 is upregulated in expression by <t>IFN</t> signaling-competent MDA5. A . Volcano plot of RNA-seq data comparing gene expression between WT and GOF MDA5 expressed HEK293 cells. B . HEK293T cells were transfected with plasmids expressing WT or GOF MDA5. 24 h later, cells were lysed for RNA detection by RT-qPCR and protein detection by western blotting. EV, empty vector. C . HEK293T cells were mock-treated or treated <t>with</t> <t>recombinant</t> IFNβ; at a final concentration of 10 ng/ml for 24 h. RNA was purified, and gene expression was tested by RT-qPCR. D . HEK293T cells were transfected with plasmids expressing MDA5. 24 h later, cells were lysed for RNA detection. The qPCR primer-targeting regions on AL590385.23 was indicated in green. E . HEK293T cells were transfected with MDA5 expressing plasmid. 6 h post transfection, cells were transfected with poly I: C at a final concentration of 0.8 ng/μ;l. 18 h later, cells were lysed for RNA measurement. F . Cells were transfected with GOF MDA5 expressing plasmid and harvested at indicated hours post transfection. RNA was measured by RT-qPCR. G , H . Act.D was added at a final concentration of 5 μ;g/ml 24 h post transfection of plasmids indicated. 24 h post treatment, cells were lysed for RNA measurement. I , J . HEK293T cells were infected with EMCV at an MOI of 0.01. 24 h post infection, cells were lysed for RNA measurement. K . RNA levels of IFNβ; and HSPA6 in epithelial cells were shown upon SARS-CoV infection. The data was obtained from GEO database (accession number: GSE17400 ). For B-E, G-J, data are means ± SD of three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001. For F, data are means ± SD of two independent experiments
Human Ifnβ, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human ifnβ/product/Sino Biological
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human ifnβ - by Bioz Stars, 2026-03
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enzyme linked immunosorbent assay elisa  (Cusabio)
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Cusabio enzyme linked immunosorbent assay elisa
HSPA6 is upregulated in expression by <t>IFN</t> signaling-competent MDA5. A . Volcano plot of RNA-seq data comparing gene expression between WT and GOF MDA5 expressed HEK293 cells. B . HEK293T cells were transfected with plasmids expressing WT or GOF MDA5. 24 h later, cells were lysed for RNA detection by RT-qPCR and protein detection by western blotting. EV, empty vector. C . HEK293T cells were mock-treated or treated <t>with</t> <t>recombinant</t> IFNβ; at a final concentration of 10 ng/ml for 24 h. RNA was purified, and gene expression was tested by RT-qPCR. D . HEK293T cells were transfected with plasmids expressing MDA5. 24 h later, cells were lysed for RNA detection. The qPCR primer-targeting regions on AL590385.23 was indicated in green. E . HEK293T cells were transfected with MDA5 expressing plasmid. 6 h post transfection, cells were transfected with poly I: C at a final concentration of 0.8 ng/μ;l. 18 h later, cells were lysed for RNA measurement. F . Cells were transfected with GOF MDA5 expressing plasmid and harvested at indicated hours post transfection. RNA was measured by RT-qPCR. G , H . Act.D was added at a final concentration of 5 μ;g/ml 24 h post transfection of plasmids indicated. 24 h post treatment, cells were lysed for RNA measurement. I , J . HEK293T cells were infected with EMCV at an MOI of 0.01. 24 h post infection, cells were lysed for RNA measurement. K . RNA levels of IFNβ; and HSPA6 in epithelial cells were shown upon SARS-CoV infection. The data was obtained from GEO database (accession number: GSE17400 ). For B-E, G-J, data are means ± SD of three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001. For F, data are means ± SD of two independent experiments
Enzyme Linked Immunosorbent Assay Elisa, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti ifn β  (MedChemExpress)
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MedChemExpress mouse anti ifn β
HSPA6 is upregulated in expression by <t>IFN</t> signaling-competent MDA5. A . Volcano plot of RNA-seq data comparing gene expression between WT and GOF MDA5 expressed HEK293 cells. B . HEK293T cells were transfected with plasmids expressing WT or GOF MDA5. 24 h later, cells were lysed for RNA detection by RT-qPCR and protein detection by western blotting. EV, empty vector. C . HEK293T cells were mock-treated or treated <t>with</t> <t>recombinant</t> IFNβ; at a final concentration of 10 ng/ml for 24 h. RNA was purified, and gene expression was tested by RT-qPCR. D . HEK293T cells were transfected with plasmids expressing MDA5. 24 h later, cells were lysed for RNA detection. The qPCR primer-targeting regions on AL590385.23 was indicated in green. E . HEK293T cells were transfected with MDA5 expressing plasmid. 6 h post transfection, cells were transfected with poly I: C at a final concentration of 0.8 ng/μ;l. 18 h later, cells were lysed for RNA measurement. F . Cells were transfected with GOF MDA5 expressing plasmid and harvested at indicated hours post transfection. RNA was measured by RT-qPCR. G , H . Act.D was added at a final concentration of 5 μ;g/ml 24 h post transfection of plasmids indicated. 24 h post treatment, cells were lysed for RNA measurement. I , J . HEK293T cells were infected with EMCV at an MOI of 0.01. 24 h post infection, cells were lysed for RNA measurement. K . RNA levels of IFNβ; and HSPA6 in epithelial cells were shown upon SARS-CoV infection. The data was obtained from GEO database (accession number: GSE17400 ). For B-E, G-J, data are means ± SD of three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001. For F, data are means ± SD of two independent experiments
Mouse Anti Ifn β, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reporter cell  (InvivoGen)
94
InvivoGen reporter cell
HSPA6 is upregulated in expression by <t>IFN</t> signaling-competent MDA5. A . Volcano plot of RNA-seq data comparing gene expression between WT and GOF MDA5 expressed HEK293 cells. B . HEK293T cells were transfected with plasmids expressing WT or GOF MDA5. 24 h later, cells were lysed for RNA detection by RT-qPCR and protein detection by western blotting. EV, empty vector. C . HEK293T cells were mock-treated or treated <t>with</t> <t>recombinant</t> IFNβ; at a final concentration of 10 ng/ml for 24 h. RNA was purified, and gene expression was tested by RT-qPCR. D . HEK293T cells were transfected with plasmids expressing MDA5. 24 h later, cells were lysed for RNA detection. The qPCR primer-targeting regions on AL590385.23 was indicated in green. E . HEK293T cells were transfected with MDA5 expressing plasmid. 6 h post transfection, cells were transfected with poly I: C at a final concentration of 0.8 ng/μ;l. 18 h later, cells were lysed for RNA measurement. F . Cells were transfected with GOF MDA5 expressing plasmid and harvested at indicated hours post transfection. RNA was measured by RT-qPCR. G , H . Act.D was added at a final concentration of 5 μ;g/ml 24 h post transfection of plasmids indicated. 24 h post treatment, cells were lysed for RNA measurement. I , J . HEK293T cells were infected with EMCV at an MOI of 0.01. 24 h post infection, cells were lysed for RNA measurement. K . RNA levels of IFNβ; and HSPA6 in epithelial cells were shown upon SARS-CoV infection. The data was obtained from GEO database (accession number: GSE17400 ). For B-E, G-J, data are means ± SD of three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001. For F, data are means ± SD of two independent experiments
Reporter Cell, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reporter cell - by Bioz Stars, 2026-03
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b16 bluetm ifn α β cells  (InvivoGen)
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InvivoGen b16 bluetm ifn α β cells
HSPA6 is upregulated in expression by <t>IFN</t> signaling-competent MDA5. A . Volcano plot of RNA-seq data comparing gene expression between WT and GOF MDA5 expressed HEK293 cells. B . HEK293T cells were transfected with plasmids expressing WT or GOF MDA5. 24 h later, cells were lysed for RNA detection by RT-qPCR and protein detection by western blotting. EV, empty vector. C . HEK293T cells were mock-treated or treated <t>with</t> <t>recombinant</t> IFNβ; at a final concentration of 10 ng/ml for 24 h. RNA was purified, and gene expression was tested by RT-qPCR. D . HEK293T cells were transfected with plasmids expressing MDA5. 24 h later, cells were lysed for RNA detection. The qPCR primer-targeting regions on AL590385.23 was indicated in green. E . HEK293T cells were transfected with MDA5 expressing plasmid. 6 h post transfection, cells were transfected with poly I: C at a final concentration of 0.8 ng/μ;l. 18 h later, cells were lysed for RNA measurement. F . Cells were transfected with GOF MDA5 expressing plasmid and harvested at indicated hours post transfection. RNA was measured by RT-qPCR. G , H . Act.D was added at a final concentration of 5 μ;g/ml 24 h post transfection of plasmids indicated. 24 h post treatment, cells were lysed for RNA measurement. I , J . HEK293T cells were infected with EMCV at an MOI of 0.01. 24 h post infection, cells were lysed for RNA measurement. K . RNA levels of IFNβ; and HSPA6 in epithelial cells were shown upon SARS-CoV infection. The data was obtained from GEO database (accession number: GSE17400 ). For B-E, G-J, data are means ± SD of three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001. For F, data are means ± SD of two independent experiments
B16 Bluetm Ifn α β Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hek blue ifn α β cells  (InvivoGen)
96
InvivoGen hek blue ifn α β cells
HSPA6 is upregulated in expression by <t>IFN</t> signaling-competent MDA5. A . Volcano plot of RNA-seq data comparing gene expression between WT and GOF MDA5 expressed HEK293 cells. B . HEK293T cells were transfected with plasmids expressing WT or GOF MDA5. 24 h later, cells were lysed for RNA detection by RT-qPCR and protein detection by western blotting. EV, empty vector. C . HEK293T cells were mock-treated or treated <t>with</t> <t>recombinant</t> IFNβ; at a final concentration of 10 ng/ml for 24 h. RNA was purified, and gene expression was tested by RT-qPCR. D . HEK293T cells were transfected with plasmids expressing MDA5. 24 h later, cells were lysed for RNA detection. The qPCR primer-targeting regions on AL590385.23 was indicated in green. E . HEK293T cells were transfected with MDA5 expressing plasmid. 6 h post transfection, cells were transfected with poly I: C at a final concentration of 0.8 ng/μ;l. 18 h later, cells were lysed for RNA measurement. F . Cells were transfected with GOF MDA5 expressing plasmid and harvested at indicated hours post transfection. RNA was measured by RT-qPCR. G , H . Act.D was added at a final concentration of 5 μ;g/ml 24 h post transfection of plasmids indicated. 24 h post treatment, cells were lysed for RNA measurement. I , J . HEK293T cells were infected with EMCV at an MOI of 0.01. 24 h post infection, cells were lysed for RNA measurement. K . RNA levels of IFNβ; and HSPA6 in epithelial cells were shown upon SARS-CoV infection. The data was obtained from GEO database (accession number: GSE17400 ). For B-E, G-J, data are means ± SD of three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001. For F, data are means ± SD of two independent experiments
Hek Blue Ifn α β Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Poxin transgene inhibits DNA sensing-induced innate immune response. A. Schematic of cloning process. B-G. Cells were transfected with plasmid DNA (pDNA) using TransIT-2020. B. 2.5 μg and 7.5 μg of pDNA encoding Poxin or mutant Poxin (mtPoxin) were transfected into 293T cells, respectively, and FLAG-tagged proteins were detected. C. Activation of STING, TBK1, and IRF3 was analyzed by western blotting. Quantitative analysis of p-IRF3 expression level is shown. D. H151 was pre-treated 1 h before pDNA transfection, and protein expression levels were assessed after 24 h. E. Cells were transfected with pEGFP-N1, and protein expression levels were detected. F. mRNA levels of IFNB1 and ISG15 were analyzed by RT-qPCR (n = 3). G. IFN-β secretion levels were quantitatively analyzed using ELISA. (Representative western blots are shown.).

Journal: Journal of Advanced Research

Article Title: Enhancement of NK cell activity via DNA-sensing inhibition by Poxin transgene

doi: 10.1016/j.jare.2025.05.058

Figure Lengend Snippet: Poxin transgene inhibits DNA sensing-induced innate immune response. A. Schematic of cloning process. B-G. Cells were transfected with plasmid DNA (pDNA) using TransIT-2020. B. 2.5 μg and 7.5 μg of pDNA encoding Poxin or mutant Poxin (mtPoxin) were transfected into 293T cells, respectively, and FLAG-tagged proteins were detected. C. Activation of STING, TBK1, and IRF3 was analyzed by western blotting. Quantitative analysis of p-IRF3 expression level is shown. D. H151 was pre-treated 1 h before pDNA transfection, and protein expression levels were assessed after 24 h. E. Cells were transfected with pEGFP-N1, and protein expression levels were detected. F. mRNA levels of IFNB1 and ISG15 were analyzed by RT-qPCR (n = 3). G. IFN-β secretion levels were quantitatively analyzed using ELISA. (Representative western blots are shown.).

Article Snippet: Quantitative analysis of secreted human IFN-β was performed following manufacturers' protocol (luex-hifnbv2, Invivogen).

Techniques: Cloning, Transfection, Plasmid Preparation, Mutagenesis, Activation Assay, Western Blot, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Poxin protects NK-92 cells from pDNA transfection-induced innate immunity. A. Cells were transfected by electroporation with 10 μg pDNA, and mRNA dynamics were analyzed by RT-qPCR (n = 3). B. IFN-β secretion levels were quantitatively analyzed using ELISA. C. Expression levels of innate immune signaling proteins were detected by western blotting. (Representative data shown). D. Quantitative analysis of p-STING and p-TBK1 expression levels.

Journal: Journal of Advanced Research

Article Title: Enhancement of NK cell activity via DNA-sensing inhibition by Poxin transgene

doi: 10.1016/j.jare.2025.05.058

Figure Lengend Snippet: Poxin protects NK-92 cells from pDNA transfection-induced innate immunity. A. Cells were transfected by electroporation with 10 μg pDNA, and mRNA dynamics were analyzed by RT-qPCR (n = 3). B. IFN-β secretion levels were quantitatively analyzed using ELISA. C. Expression levels of innate immune signaling proteins were detected by western blotting. (Representative data shown). D. Quantitative analysis of p-STING and p-TBK1 expression levels.

Article Snippet: Quantitative analysis of secreted human IFN-β was performed following manufacturers' protocol (luex-hifnbv2, Invivogen).

Techniques: Transfection, Electroporation, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot

HSPA6 is upregulated in expression by IFN signaling-competent MDA5. A . Volcano plot of RNA-seq data comparing gene expression between WT and GOF MDA5 expressed HEK293 cells. B . HEK293T cells were transfected with plasmids expressing WT or GOF MDA5. 24 h later, cells were lysed for RNA detection by RT-qPCR and protein detection by western blotting. EV, empty vector. C . HEK293T cells were mock-treated or treated with recombinant IFNβ; at a final concentration of 10 ng/ml for 24 h. RNA was purified, and gene expression was tested by RT-qPCR. D . HEK293T cells were transfected with plasmids expressing MDA5. 24 h later, cells were lysed for RNA detection. The qPCR primer-targeting regions on AL590385.23 was indicated in green. E . HEK293T cells were transfected with MDA5 expressing plasmid. 6 h post transfection, cells were transfected with poly I: C at a final concentration of 0.8 ng/μ;l. 18 h later, cells were lysed for RNA measurement. F . Cells were transfected with GOF MDA5 expressing plasmid and harvested at indicated hours post transfection. RNA was measured by RT-qPCR. G , H . Act.D was added at a final concentration of 5 μ;g/ml 24 h post transfection of plasmids indicated. 24 h post treatment, cells were lysed for RNA measurement. I , J . HEK293T cells were infected with EMCV at an MOI of 0.01. 24 h post infection, cells were lysed for RNA measurement. K . RNA levels of IFNβ; and HSPA6 in epithelial cells were shown upon SARS-CoV infection. The data was obtained from GEO database (accession number: GSE17400 ). For B-E, G-J, data are means ± SD of three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001. For F, data are means ± SD of two independent experiments

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: HSPA6 is induced by RIG-I-like receptors and negatively regulates type-I interferon signaling

doi: 10.1007/s00018-026-06147-8

Figure Lengend Snippet: HSPA6 is upregulated in expression by IFN signaling-competent MDA5. A . Volcano plot of RNA-seq data comparing gene expression between WT and GOF MDA5 expressed HEK293 cells. B . HEK293T cells were transfected with plasmids expressing WT or GOF MDA5. 24 h later, cells were lysed for RNA detection by RT-qPCR and protein detection by western blotting. EV, empty vector. C . HEK293T cells were mock-treated or treated with recombinant IFNβ; at a final concentration of 10 ng/ml for 24 h. RNA was purified, and gene expression was tested by RT-qPCR. D . HEK293T cells were transfected with plasmids expressing MDA5. 24 h later, cells were lysed for RNA detection. The qPCR primer-targeting regions on AL590385.23 was indicated in green. E . HEK293T cells were transfected with MDA5 expressing plasmid. 6 h post transfection, cells were transfected with poly I: C at a final concentration of 0.8 ng/μ;l. 18 h later, cells were lysed for RNA measurement. F . Cells were transfected with GOF MDA5 expressing plasmid and harvested at indicated hours post transfection. RNA was measured by RT-qPCR. G , H . Act.D was added at a final concentration of 5 μ;g/ml 24 h post transfection of plasmids indicated. 24 h post treatment, cells were lysed for RNA measurement. I , J . HEK293T cells were infected with EMCV at an MOI of 0.01. 24 h post infection, cells were lysed for RNA measurement. K . RNA levels of IFNβ; and HSPA6 in epithelial cells were shown upon SARS-CoV infection. The data was obtained from GEO database (accession number: GSE17400 ). For B-E, G-J, data are means ± SD of three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001. For F, data are means ± SD of two independent experiments

Article Snippet: The recombinant human IFNβ (Sino Biological, 10704-HNAS) was dissolved in PBS at a stock concentration of 1.0 mg/ml as the manufacture’s instruction suggested.

Techniques: Expressing, RNA Sequencing, Gene Expression, Transfection, RNA Detection, Quantitative RT-PCR, Western Blot, Plasmid Preparation, Recombinant, Concentration Assay, Purification, Infection